Haplotipos mitocondriales de Phytophthora andina de tomate de árbol en el Ecuador

The mitochondrial DNA haplotype of 131 pure cultures of Phytophthora andina collected from tree tomato (Solanum betaceum) in several provinces in Ecuador was determined. The P2 and P4 regions of the mtDNA were amplified, and the PCR products obtained were digested with MspI and EcoRI restriction enzymes. All isolates analyzed showed a band pattern corresponding to the Ia haplotype for P. infestans, that is, bands of sizes 720 and 350 bp with MspI (P2 region) and bands of sizes 394, 361 and 309 bp with EcoRI (P4 region). However, additional bands that have not been reported previously were also consistently observed in all isolates analyzed. For the P2 region, additional bands of 400 and 500 bp and an undigested fragment of 1070 bp were obtained, and for the P4 region, additional bands of 603 and 755 bp in addition to an undigested fragment of 964 bp. Finding new band patterns in a consistent manner for all analyzed isolates of P. andina suggests that the description of haplotype Ia assigned to P. andina from tree tomato should be redefined.