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1658-6794© 2020. AJFSFM. This is an open access article, distributed under the terms of the Creative Commons, Attribution-NonCommercial License. Abstract Short Tandem Repeats (STR) have been widely used to create a discriminating DNA profile during the forensic investigation of a crime. The Paternity and Kinship Division at Medico-legal Directorate (MLD)/ Baghdad, provides a DNA fingerprinting service for paternity and kinship requests from different courts of law and police departments from across the country. Several rare variants were observed during DNA analysis such as rare off-ladder alleles, tri-allelic pattern as well as allele dropout. Variants that have been transmitted among family members were investigated in this study. During the period between 2008 to 2019, 38309 samples have been analyzed in the Paternity and Kinship Laboratories for DNA profiling to resolve the referred cases. DNA profiles found to have unusual STR patterns (off-ladder, tri-allelic pattern, inter-loci variable were analyzed and documented). A total of 17 variants were observed which were as shared among family members. Rare off-ladder alleles (9 cases + inter-loci variant 2 cases), as well as 6 cases of ضلختسلما ىلع متي )STR( ةرركتلما ةيرصقلا تاعباتتلا مادختصا نإا لامج نمص ةزيمم ةيثارو ةمصب ىلع لوصلحا ضرغل عصاو قاطن بطلا ةرئاد في بصنلاو ةيدئاعلا ةبعص ىنعتو .ةيئانلجا تاصوحفلا نم بصنلاو ةوبألا اياصقل يوونلا ضملحا تاصوحفب دادغبب ليدعلا تمو .دلابلا ءاحنأا عيمج نم ةطرصلا تارادإاو نوناقلا مكامح فلتمخ لثم( يوونلا ضملحا ليلتح ءانثأا تايرغتلما نم عاونأا ةدع ليجصت ,ةددعتم عقاومو ,تلايلألا يثلاث طنمو ,ملصلا جراخ ةديدج تلايلأا ينب ةكترصلما تارياغتلا ضحف تمو ,)ليلألا برصت لىإا ةفاصإلاب .ةصاردلا هذه نمص ةرصألا دارفأا لوصحلل بصنلاو ةيدئاعلا تابرتمخ في ةنيع 38309 ليلتح تمو للاخ ةبعصلل ةلاحلما اياصقلا لح ضرغل يوونلا ضمالحا ةمصب ىلع ضمالحا تامصب ليلتحو قيثوت تمو .2019 لىإا 2008 ينب ةترفلا ,ملصلا جراخ( ةيداع يرغ STR طانمأا اهيدل نأا تدجو يتلا يوونلا .ةصاردلا هذه نمص )تلايلألا يثلاث طنمو عقاولما ينب تلايلأا ةكترصم اهنأا ىلع اهفاصتكاو ايًرغتم 17 هعوممج ام دصر تم امك عصت( ملصلا جراخ ةيرغتلما ةديدلجا تلايلألاو ,ةرصألا دارفأا ينب Arab Journal of Forensic Sciences & Forensic Medicine 2020; Volume 2 Issue (2), 111-117 Original Article

arab journal of forensic sciences and forensic medicine

Description of Rare Genetic Variants Discovered with Promega PowerPlex® Forensic Amplification Kits during STR analysis of Routine Paternity and Kinship Cases