Open AccessRELIABILITY OF QUANTIFICATION OF CANDIDA SPECIES COLONIES USING ALTERNATIVE PLATING METHODS
Author(s) -
Sarah Raquel de Annunzio,
Filipe Silveira Fusco,
Carolina Santezi,
Bárbara Donadon Reina,
Lívia Nordi Dovigo
Publication year - 2019
Publication title -
revista prevenção de infecção e saúde
Language(s) - English
Resource type - Journals
ISSN - 2446-7901
DOI - 10.26694/repis.v6i0.9426
Subject(s) - candida tropicalis , candida krusei , candida albicans , intraclass correlation , candida glabrata , serial dilution , concordance , microbiology and biotechnology , corpus albicans , chromatography , biology , chemistry , reproducibility , medicine , bioinformatics , pathology , alternative medicine
Objective: to evaluate the concordance of different plating methods for quantification of Candida species colonies. Method: standardized suspensions of reference strains (Candida albicans, Candida glabrata, Candida tropicalis and Candida krusei) were submitted to serial dilution and plating according to methods of track-dilution (TDM), drop plate (DPM) and the conventional spread plate (SPM). Data were submitted to construction of Bland–Altman diagrams, Intraclass Correlation Coefficient (ICC) and ANOVA (⍺=5%). Results: adequate concordance between the methods (CCI >0.71) was observed, and the execution of DPM was the fastest (p<0.001). However, DPM and TDM appear to result in greater values compared to SPM, especially for C. tropicalis and C. krusei. Conclusion: C. albicans and C. glabrata can be plated with DPM and TDM, but the use of these methods for C. krusei and C. tropicalis may result in count variation.
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