ANTIMICROBIAL AND SYNERGISTIC ACTIVITY OF ESSENTIAL OILS FACING ISOLATED BACTERIA FROM SURGICAL STAFF’S
Author(s) -
Tatiana Areas da Cruz,
Fernanda Rodrigues Torres,
Monise Fazolin Petrucelli,
Mariana Heinzen de Abreu,
Silvia Sidneia Silva,
Mozart Marins Marins,
Renê Oliveira Beleboni,
Ana Lúcia Fachin
Publication year - 2019
Publication title -
revista prevenção de infecção e saúde
Language(s) - English
Resource type - Journals
ISSN - 2446-7901
DOI - 10.26694/repis.v5i0.8970
Subject(s) - proteus vulgaris , antimicrobial , staphylococcus epidermidis , minimum inhibitory concentration , essential oil , staphylococcus aureus , microbiology and biotechnology , broth microdilution , bacteria , staphylococcus saprophyticus , proteus , microorganism , salmonella , food science , chemistry , biology , escherichia coli , biochemistry , gene , genetics
Objective: to evaluate antimicrobial and synergistic activity of essential oils ( Citrus aurantifolia, Citrus sinensis, Mentha viridis and Thymus vulgaris ) against isolated bacteria from surgical staff’s hands and bacterial strains. Method: the antimicrobial activity of oils was analyzed by microdilution method to determine the Minimum Inhibitory Concentration. Moreover, the combinatory effect of the oil that presented greater effectiveness using gentamycin sulphate through the Fractional Inhibitory Concentration index was tested. The study was performed over the period 2016-2018. Results: T. vulgaris was effective against Staphylococcus saprophyticus with a concentration of 0.0008 µL.mL -1 and against the ATCC standard strains of Staphylococcus aureus , Salmonella choleraesuis, Staphylococcus epidermidis and Proteus vulgaris and the bacteria S. aureus , S. epidermidis and Bacillus sp . with MIC ≥ 0.10 µL.mL -1 . This oil association with gentamycin sulphate showed synergistic activity against S. epidermidis. Conclusion: T. vulgaris essential oil showed expressive antimicrobial activity against ATCC and isolated clinic microorganisms, suggesting broad spectrum of activity.
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