BACTERIOPHAGE ISOLATION FROM DENTURE BIOFILM

Denture biofilm represents a potential source of microbial contamination/infection that has been combated by new hygiene methods. Bacteriophages have already related as a biofilm growth controller, but dentures research is scarce. Objective: this research evaluated if it is possible to isolate bacteriophages from dentures biofilm, and use it as an emergent way to control biofilm growing. Method: biofilm samples were collected from sixteen maxillary complete dentures in an aseptic zone by brushing their internal surfaces using a soft toothbrush. The biofilm suspensions obtained were added to standard strains ( Staphylococcus aureus , Streptococcus mutans , Enterococcus faecalis , Escherichia coli and Pseudomonas aeruginosa ) and the presence of lysis was evaluated. Results: bacteriophages were not isolated from the denture samples. Conclusion: this study presented an opportunity to invest in a new method to detect bacteriophages from mature biofilms of complete dentures.