Exposição combinada de desreguladores endócrinos : efeitos do bisfenol a em camudongos sensitizados por 4- nonilfenol na fase perinatal

Endocrine disruptors are substances capable of affecting the reproductive systems, the secretion and the hormones metabolism through the effects on the synthesis. Among these disruptors, it can be highlighted the 4-Nonylphenol (NP) and the bisphenol A (BFA), they are known for binding to the estrogen receptors and for interfering on these hormones synthesis and also on the progesterone synthesis. Since the estrogen and the progesterone are involved on the cell proliferation on the mammary gland and endometrium tissues, this research aimed to evaluate the effects of the exposure to low doses of 4-nonyphenol and Bisphenol A in breast and endometrial tissue of mice. For such, Balb/C female mice received daily, by gavage, during pregnancy and lactation, NP solubilized in corn oil at a concentration of 50 mg/Kg or else corn oil or water. The offspring of females that received water or corn oil, after weaning, started receiving the same as their mothers, constituting the Water/Water and Oil/Oil group, respectively. The offspring of females that received NP, after weaning, started to receive water, corn oil or Bisphenol A solubilized in corn oil at a concentration of 25mcg/Kg, thus constituting, respectively, the NP/Water, NP/Oil and NP/BFA groups. Initially, it was evaluated whether the exposure to Nonyphenol affected the fertility rate and the male/female ratio among newborns. Thereafter, it was evaluated in the offspring the possible toxicity of NP and BFA dosing to the serum levels of the enzymes alkaline phosphatase, gamma glutamyltransferase (yGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatinine levels, urea, and hormones involved in reproduction regulation. The determination of toxicity also involves morphological examination of organs such as liver, kidney and spleen. The effect of exposure to Nonylphenol and to Bisphenol A in breast and endometrial tissues was evaluated by morphological