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Over the past 20 years, the use of monoclonal antibodies for therapeutic purposes has been a great ally for the treatment of various diseases. Anti-CD3 antibodies are used in the prevention of transplant organs and have the potential to treat autoimmune diseases. However, the use of these antibodies, although efficient generate an immune response because most of them have murine origin, when administered preventing the frequent use, such as OKT3. The humanization of antibodies, which is performed by means of gene manipulation, it has been a practical solution to this problem, reducing the immunogenic response elicited by the use of murine antibodies. From a human antibody library constructed from a phage display technique it is possible to build new human antibodies with the highest binding efficiency resulting in less adverse effects in helping these clinical diseases and improving the quality of life of patients. In this study, from a synthetic construct scFvRVL_gen3 containing humanized VH and VL gene and the coding sequence of the protein 3 (gene 3) was constructed using a plasmid vector pCIgRM (plasmid containing anti-CD3 murine VH and VL), resulting 4 positive clones for pCIgRVL_G3 construction. For amplification of VL of the human antibody gene library in vector pComb 3X after the 23 different test systems PCR reactions, thereby optimizing the conditions has been established for it to happen. The product was then cloned into the vector pGEM®T to study the efficiency of the amplification reaction and library diversity. The data obtained in this study are useful tools for cloning of various sequences of the amplified VL pCIgRVL_G3 VL vector by replacing the synthetic human VL library, thus allowing step forward for the construction of a human monoclonal anti-CD3 antibody.

Construção in vitro de bibliotecas de cadeias leves humanas para seleção de novos anticorpos anti-CD3