Efeitos do inibidor de proteases Black-Eyed Pea Trypsin Chymotrypsin Inhibitor (BTCI) na viabilidade e proliferação de células MDA-MB-231 de adenocarcinoma mamário

The mammary adenocarcinoma as well as other manifestations of cancer, is the pathological phenotype triggered by multifactorial processes and multiprocessual development, due to proliferation and cell growth control changes. In this study the effects of the black-eyed pea trypsin chymotrypsin protease inhibitor (BTCI) were evaluated in breast adenocarcinoma cell (MDA-MB-231) proliferation and feasibility in vitro, in order to characterize the BTCI as a potential anticarcinogenic agent for this type of cancer cell. Thus viability assays, assessments of cell cycle and cell death by flow cytometry, as well as study of the generation of reactive oxygen molecular species (ROS) were performed. Upon determining the inhibitory concentration (IC50) of BTCI (267.4 uM in 24 hours) the interference of the inhibitor on cell cycle was evaluated and an increased number of cells in the G2 phase was observed, as well as presence of fragmented DNA. The death pathway of mammary adenocarcinoma cells treated with BTCI was studied and it was found that the average of the cells that entered death process by apoptosis was 89.4%, significant value p<0.001. Furthermore, cell oxidation process via Oxigen Reactive Species (ROS) was intensified after 24 hours of incubation. The results indicate that BTCI has cytostatic and cytotoxic effects on the analyzed cells. The main pathway of cell death was apoptosis, a process that may be associated with cellular oxidation by oxygen free radicals.