Identification of Streptococcus agalactiae isolated from pregnant women by 16srRNA gene | Zendy

Haneen kadhum Abdul-Hamza | Zendy; Ghaidaa Jihadi Mohammed | Zendy

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Identification of Streptococcus agalactiae isolated from pregnant women by 16srRNA gene

Author(s) -

Haneen kadhum Abdul-Hamza,

Ghaidaa Jihadi Mohammed

Publication year - 2019

Publication title -

international journal of research in pharmaceutical sciences

Language(s) - English

Resource type - Journals

ISSN - 0975-7538

DOI - 10.26452/ijrps.v10i2.783

Subject(s) - streptococcus agalactiae , microbiology and biotechnology , polymerase chain reaction , biology , gene , streptococcus , gene sequence , bacteria , 16s ribosomal rna , genetics

Received on: 16.11.2018 Revised on: 18.03.2019 Accepted on: 22.03.2019 The main goal of the current study is to isolate and diagnose of Streptococcus agalactiae by using the diagnostic 16 SrRNA gene. S.agalactiae was isolated from 850 samples including (425) vaginal swabs, (425) rectal swabs and identified by studying the morphological characteristics of colonies on culture, microscopic characteristics of bacterial cells, biochemical methods, Vitek 2 system, API-20 Strep, and then confirmed the identification by detection of 16SrRNA gene by Polymerase Chain reaction (PCR) followed by DNA Sequence analysis for this gene. A total of 16 isolates of S.agalactiae were isolated from 12 (2.82%) vaginal swabs, 4 (0.99%) rectal swabs, and 16SrRNA was detected in 100% of the S.agalactiae isolates.

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