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Received on: 19.10.2018 Revised on: 09.03.2019 Accepted on: 12.03.2019 Nitrogen-containing, heterocycles, have special importance and vital, role in the discovery of effective bioactive, agents in the pharmaceutical, industry. The present article reports the synthesis of new fused, heterocycles triazolotetrazine by cyclo condensation, reaction as shown in scheme(1). The structures formula of synthesized compounds newly was evaluated by Ft-IR,1HNMR spectrum, and C, H, N elemental analysis. Antimicrobial activity of triazolotetrazine studied against some pathogenic bacterial strains isolated from patients like Acinetobacter, Aeromonas, E. coli, Klebsiella, Staphylococcus, Streptococcus. Eventually, antibacterial of the fused heterocyclic compound was exhibited significant growth inhibition against some pathogenic bacteria which consider an important source of new antimicrobial compounds. The results, of such studies, are discussed in this paper.

Synthesis and antimicrobial evaluation of fused heterocyclic compound [1,2,4] triazolo [4,3-b][1,2,4,5] tetra zine