Identification of a novel microRNA important for melanogenesis in alpaca (Vicugna pacos)1 | Zendy

Shanshan Yang | Zendy; Ruiwen Fan | Zendy; Zeyu Shi | Zendy; Kaiyuan Ji | Zendy; Junxing Zhang | Zendy; H. Wang | Zendy; Muren Herrid | Zendy; Qiaoqiao Zhang | Zendy; Jianbo Yao | Zendy; G. W. Smith | Zendy; Changsheng Dong | Zendy

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Identification of a novel microRNA important for melanogenesis in alpaca (Vicugna pacos)1

Author(s) -

Shanshan Yang,

Ruiwen Fan,

Zeyu Shi,

Kaiyuan Ji,

Junxing Zhang,

H. Wang,

Muren Herrid,

Qiaoqiao Zhang,

Jianbo Yao,

G. W. Smith,

Changsheng Dong

Publication year - 2015

Publication title -

journal of animal science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.928

H-Index - 156

eISSN - 1525-3015

pISSN - 0021-8812

DOI - 10.2527/jas.2014-8404

Subject(s) - microphthalmia associated transcription factor , biology , tyrosinase , microrna , melanin , gene , downregulation and upregulation , microbiology and biotechnology , guanosine , cyclic adenosine monophosphate , messenger rna , gene knockdown , coat , gene expression , transcription factor , biochemistry , receptor , enzyme , paleontology

The molecular mechanisms underlying the formation of coat colors in animals are poorly understood. Recent studies have demonstrated that microRNA play important roles in the control of melanogenesis and coat color in mammals. In a previous study, we characterized the miRNA expression profiles in alpaca skin with brown and white coat color and identified a novel miRNA (named lpa-miR-nov-66) that is expressed significantly higher in white skin compared to brown skin. The present study was conducted to determine the functional roles of this novel miRNA in the regulation of melanogenesis in alpaca melanocytes. lpa-miR-nov-66 is predicted to target the soluble guanylate cyclase (sGC) gene based on presence of a binding site in the sGC coding sequence (CDS). Overexpression of lpa-miR-nov-66 in alpaca melanocyes upregulated the expression of sGC both at the mRNA and protein level. Overexpression of lpa-miR-nov-66 in melanocyes also resulted in decreased expression of key melanogenic genes including tyrosinase (TYR), tyrosinase related protein 1 (TYRP1), and microphthalmia transcription factor (MITF). Our ELISA assays showed increased cyclic guanosine monophosphate (cGMP) but decreased cyclic adenosine monophosphate (cAMP) production in melanocytes overexpressing lpa-miR-nov-66. In addition, overexpression of lpa-miR-nov-66 also reduced melanin production in cultured melanocytes. Results support a role of lpa-miR-nov-66 in melanocytes by directly or indirectly targeting , which regulates melanogenesis via the cAMP pathway.

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