Rapid communication: genetic linkage and physical mapping of the porcine cholesteryl ester transfer protein (CETP) gene1

Source and Description of Primers. The conserved sequences of human and rabbit cholesteryl ester transfer protein gene (CETP) (GenBank accession numbers NM000078 and M27486, respectively) were used to design primers to PCR amplify porcine CETP. The primers amplified a 970-bp fragment of porcine CETP gene spanning an intron between exon 1 and exon 2. The porcine CETP sequence (GenBank accession number AF333037) determined from this fragment showed 80.9%(34/42) and 78.6% (33/42) nucleotide identity to the corresponding human and rabbit CETP exon 2 sequence, respectively. To physically map porcine CETP by PCR testing pig/ rodent somatic cell hybrid panel (SCHP) and to develop PCR-RFLP marker for linkage mapping in PigMaP reference families, a pig-specific reverse primer was designed based on the new generated porcine intron 1 sequence.