Rapid communication: restriction fragment length polymorphisms at the porcine transporter associated with antigen processing 1 (TAP1) locus2 | Zendy

D. A. Vaske | Zendy; Marja Ruohonen-Lehto | Zendy; R. G. Larson | Zendy; Carol M. Warner | Zendy; M. F. Rothschild | Zendy

Open Access

Rapid communication: restriction fragment length polymorphisms at the porcine transporter associated with antigen processing 1 (TAP1) locus2

Author(s) -

D. A. Vaske,

Marja Ruohonen-Lehto,

R. G. Larson,

Carol M. Warner,

M. F. Rothschild

Publication year - 1994

Publication title -

journal of animal science

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.928

H-Index - 156

eISSN - 1525-3015

pISSN - 0021-8812

DOI - 10.2527/1994.721255x

Subject(s) - rothschild , library science , political science , computer science , law

Source and Description of Probe. A 2.7-kb human cDNA clone for TAP1 was excised from the XbaI site of the plasmid pRSV.5neo (Spies et al., 1990). Method of Detection. Hybridizations were performed at 65°C for 16 to 20 h (10% dextran sulfate, 7% SDS, .263 M NazHP04, 1% BSA, 1 mM EDTA, 100 pg/mL sonicated denatured salmon sperm DNA). Final washes were done at 65°C in .7x SSC, .5% SDS for 15 to 20 min.

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