Methanolic Extract of Mucuna pruriens Seed Acts for Neuroprotection and Antioxidant Defense in A Fish Model

The anti-oxidative and neuroprotective effects of Mucuna pruriens seeds and L-Dopa were evaluated in Nile tilapia. Fish were divided into five groups (n=30, 2 replicates). One group was fed control diet, one with diet containing methanolic M. pruriens seed extract (0.2 g/kg feed) and one with diet containing L-Dopa (0.034gm/kg) for 30 days. The 4 and 5 group of fish were fed control diet but injected with Mucuna extract (0.015gm/mL) and L-Dopa (0.0008gm/mL), respectively, once daily for three days from 27 to 30 day of the feeding period. After 30 days, levels of different enzymatic and non-enzymatic antioxidants, acetylcholinesterase (AChE), monoamine oxidase (MAO), nitric oxide (NO), H2O2 protection, hydroxyl radical scavenging, alkaline phosphatase (ALP), glutamic oxaloacetic transaminase (GOT) and glutamic pyruvate transaminase (GPT) activities were measured in brain tissue and serum. There was significant (P<0.05) increase in AChE, H2O2 protection, MAO, superoxide dismutase, glutathione S-transferase, glutathione and glutathione reductase activities in all four treatment groups compared to those in control fish. Glutathione peroxidase, ALP and GPT levels decreased significantly (P<0.05) in all treatment groups than those in control.