Chemical Constituents and Antioxidant Activity of a Polar Extract from Pituranthos battandieri Maire

Phytochemical investigation of the n-butanol soluble part of the aqueous-MeOH extract of the aerial parts of Pituranthos battandieri Maire collected from the region of Bechar in the south-west of Algeria, led to the isolation and structural elucidation of mannitol 1, xanthotoxol 2, 8-(6’,7’-dihydroxygeranyloxy)-psoralen 3 and isorhamnetin 3-O-ß-Dglucopyranoside (cacticin) 4. The structures were established by spectral analyses, mainly ESI-HRMS, UV and NMR experiments (H, C, DEPT, COSY, HSQC and HMBC) and comparison with literature data. All these compounds were described for the first time from this endemic species. The free radical scavenging activity of this extract was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging activity models. Antihemolytic property was performed using H2O2 induced red blood cell hemolysis model. Antiperoxidative effect was evaluated using lipid peroxidation-ammonium thiocyanate model. Both DPPH and H2O2 assays indicated antioxidant ability of n-BuOH extract of P. battandieri (BEPB), that were reflected by IC50 values 876.16±7.96 μg/mL and 603.8±71.06 μg/mL, respectively. In addition, the results of the present study clearly indicated that BEPB inhibited rat erythrocytes hemolysis induced by H2O2 in a dose dependent way with IC50 value 782.38±89.97 μg/mL. The inhibition of lipid peroxides generated from linoleic acid was recorded at IC50: 496.04±117.06 μg/mL. The antiperoxidative and the antihemolytic activities of P. battandieri appear as a consequence of synergistic interactions between active constituents among them mannitol 1, the furanocoumarins 2 and 3, and the flavonoid 4.