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Justicia gendarussa Burm.f. has become important source of β-sitosterol which is associated with other phenolic,terpenoids,alkaloids and steroids. Plant sterols show anti-inflammatory activity. β-sitosterol is one of phytosterol, in a mouse model of acute inflammation, and β-sitosterol effect on leukocyte recruitment, cytokines levels, and oxidative stress. The anti-inflammatory activities of β-sitosterol were assessed by measuring paw edema induced by different inflammatory agents. It separated from Justicia gendarussa burm.f. and characterization of β-sitosterol carried out by IR, NMR, and mass spectrometry. β-sitosterol shows potent as Anti-inflammatory activity by releasing histamine (30.07%), serotin and bradykinin (52.25%), and prostaglandin (69.43%) as compared to standard (Dicolfenac 5mg/kg). Objectives:To isolate, separate and characterization of β-sitosterol and to evaluate the anti-inflammatory activity of β-sitosterol extracted from Chloroform extract of Justicia Gendarussa Burm.f.

Isolation and Characterization of β-Sitosterol from Justicia gendarussa burm. F.-An Anti-Inflammatory Compound