Genetic Sequence of Drug-Resistant Haemophilus influenza Isolated from Respiratory Tract Infections

This work was done during the period from 1st May 2019 to 15th January 2020. By using the concentration of the inhibitor that was carried out for the Haemophilus influenza bacteria, isolates were identified that 50 samples of bacterial colonies of these bacteria, it was found that all isolates were resistant to ampicillin, cefuroxime, pipracilline, cefotoxone, erythromycin, and azithromycin. The counter-current immunoelectrophoresis (CCIE) test and the latex agglutination method have been employed for this purpose. These tests have aided in establishing a rapid diagnosis of H. influenzae type b infections in some cases, the positive isolates were identified for those infected with the bacteria were CCIE 7/10, 3/3, 0/5, 0/1, and 0/4, while the co-agglutination was 6/10, 3/3, 0/5, 0/1, and 0/4. The standard phenol/ chloroform extraction was used to purify DNA from the sputum’s pellet, followed by precipitation of ethanol. Each reaction set involved sterile water in place of DNA as a negative control. There is a change in the genetic sequence of drug-resistant isolates, CTT to CTA and TTT to TAT.