Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria | Zendy

Hiba H. R. | Zendy; Eesa. M. Thalij | Zendy; Sundus J. M. | Zendy

Open Access

Demodulation and purification of ellagic acid from pomegranate fruit and study antimicrobial activity against the pathogenic bacteria

Author(s) -

Hiba H. R.,

Eesa. M. Thalij,

Sundus J. M.

Publication year - 2019

Publication title -

tikrit journal of pure science

Language(s) - English

Resource type - Journals

eISSN - 2415-1726

pISSN - 1813-1662

DOI - 10.25130/j.v24i6.887

Subject(s) - ellagic acid , antimicrobial , chromatography , chemistry , extraction (chemistry) , staphylococcus aureus , pathogenic bacteria , bacteria , antibacterial activity , silica gel , high performance liquid chromatography , escherichia coli , microbiology and biotechnology , biology , biochemistry , organic chemistry , polyphenol , antioxidant , genetics , gene

extraction method. This method includes extraction with 20% (v/v) methanol under reflux conditions for purification of acid by preparative TLC method using glass plates (20 × 20 cm) coated with silica gel .This acid was detected by TLC, HPLC, UV methods and functional group of the acidwhich was extracted by FTIR technique. The antibacterial activity of Ellagic acid extract of pomegranate fruit powder was defined by petri Dish technique versus different microbial types, Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli, Pseudomonas aeruginosa) and two Yeasts(C. glabrata and C. albicams).

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