Improving in vitro seed sprouting on legume of Indigofera zollingeriana stored seed

Experimental evidence shows that notable Indonesian forage crop zollinger blue or Indigofera zollingeriana has high seed dormancy that hinders its reproduction on large scale.  This study reports different pre-treatments to break seed dormancy and improve seed germination of I. zollingeriana seeds under in vitro conditions.  Experimental evidence suggest that both mechanical and chemical scarification followed by treatment with constantly agitated liquid 0.11 mg/L GA3 has significant effects on seed germination of the plant. The germinated seeds were cultured on MS medium to aid seedling growth.  The results showed improved germination and raising of the seedlings compared to the treatments; when the seedlings were germinated using sand paper or acid scarified seeds singly. However, acid scarification for longer time affect negatively on germination especially roots.