Mikropropagasi Tangkai Daun Iles-Iles (Amorphophallus muelleri Blume) Secara In Vitro dengan Penambahan ZPT BAP dan NAA

Iles-iles (Amorphophallus muelleri Blume ) is a tuber plant belongs to the Araceae which has profit to be food diversification. Cultivated of iles-iles need long time, so an alternative cultivation by tissue culture needed. The research aims to know concentration of plant growth regulator BAP and NAA exactly for micropropagation leaf petioles of iles-iles. The research was conducted for seven months from August 2016 to February 2017. The place of research was in the Tissue Culture Laboratory, State Polytechnic of Jember. This study uses a completely randomize design (CRD) with 2 factors, each factors contain 2 level, and 6 replications. The first factor is BAP consists of three levels: 1, 2, and 3 mg/L. The second factor is NAA consist of three levels: 0.1, 0.2, and 0.3 mg/L. Data were analyzed by using Analysis of Variance, LSD level 5% and 1%. The experiment showed that the addition of 1 mg/L and 2 mg/L BAP gave effect in parameters time of callus induction, quantity of callus and time of root induction. Treatment 0,1 mg/L NAA gave effect in parameter shoot height at 63 DAP. The interaction between 1 mg/L BAP and 0,1 mg/L NAA gave effect in parameters shoot total at 63 DAP and shoot height at 63 DAP. The experiment showed that implementation of different plant growth regulator between BAP and NAA caused response on micropropagation leaf petioles of iles-iles especially in shoot formation.