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This study was performed to determine the percentage of hatchability, post-hatch growth performance, and immune response of broiler subjected to in-ovo injection of some natural oils. A total of 360 eggs of ross broiler strain were obtained. Eggs were distributed into six groups: T1,Negative Control (No injection), T2, positive control (injected with 0.1 ml distilled water; T3 injected with 0.1ml of rosemary oil ; T4, injected with 0.1ml of black seed oil; T5 injected with 0.1 ml of olive oil, and T6, injected with 0.1 ml of almond oil. The newly hatched chicks were distributed into 6 groups of 4 replicates of 10 chick. Higher hatchability percentage was found in rosemary oil, followed by almond oil and olive oil –injected eggs. The final weights of birds that were hatched from rosemary oil, and black seed oil -injected groups were significantly (P < 0.05) higher than other experimental groups. Feed intake and feed conversion ratio were improved with in -ovo administration of rosemary oil and black seed oil. In addition, the level of blood cholesterol and blood glucose were decreased (p<0.05) in birds from all-natural oils-injected eggs. The level of IgM and IgG was significantly (p<0.05) increased in the serum of birds that were hatched from natural oils injected eggs. It could be concluded that in-ove injection of natural oils used in this study was promising to increase the hatchability percentage and improve the subsequent growth and immune responses of post hatched birds.

Effect of in-ovo injection of some natural oils on the hatchability and subsequent physiological responses of post hatch broilers