Open AccessDetection of microparticles from human red blood cells by multiparametric flow cytometry.
Author(s) -
Giulia Grisendi,
Elena Finetti,
Daniele Manganaro,
Nicoletta Cordova,
Giuliano Montagnani,
Carlotta Spano,
Malvina Prapa,
Valentina Guarneri,
Satoru Otsuru,
Edwin M. Horwitz,
Giorgio Mari,
Massimo Dominici
Publication year - 2015
Publication title -
pubmed
Language(s) - English
Resource type - Journals
eISSN - 2385-2070
pISSN - 1723-2007
DOI - 10.2450/2014.0136-14
Subject(s) - glycophorin , flow cytometry , annexin , haemolysis , staining , microparticle , hemolysis , chemistry , annexin a5 , leukoreduction , microbiology and biotechnology , red blood cell , antibody , cytometry , andrology , immunology , biology , blood transfusion , biochemistry , pathology , medicine , membrane , astrobiology
During storage, red blood cells (RBC) undergo chemical and biochemical changes referred to as "storage lesions". These events determine the loss of RBC integrity, resulting in lysis and release of microparticles. There is growing evidence of the clinical importance of microparticles and their role in blood transfusion-related side effects and pathogen transmission. Flow cytometry is currently one of the most common techniques used to quantify and characterise microparticles. Here we propose multiparametric staining to monitor and quantify the dynamic release of microparticles by stored human RBC.
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