Open AccessA new strategy to identify rare blood donors: single polymerase chain reaction multiplex SNaPshot reaction for detection of 16 blood group alleles.
Author(s) -
Flávia Roche Moreira Latini,
Diana Gazito,
Carine Prisco Ari,
Janaína Guilhem Muniz,
Rosangela de Medeiros Person,
Fabrício Oliveira Carvalho,
Wilson Baleotti,
Lilian Castilho,
José Augusto Barreto
Publication year - 2014
Publication title -
pubmed
Language(s) - English
Resource type - Journals
eISSN - 2385-2070
pISSN - 1723-2007
DOI - 10.2450/2013.0242-12
Subject(s) - genotyping , multiplex , polymerase chain reaction , biology , multiplex polymerase chain reaction , genotype , typing , allele , single nucleotide polymorphism , microbiology and biotechnology , genetics , genomic dna , dna , gene
As an alternative to phenotyping, large-scale DNA-based assays, which are feasible for high-throughput donor red blood cell typing, were developed for determination of blood group polymorphisms. However, high-throughput genotyping platforms based on these technologies are still expensive and the inclusion of single nucleotide polymorphisms and analysis of the alleles depend on the manufacturer's determination. To overcome this limitation and in order to develop an assay to enable the screening of rare donors, we developed a SNaPshot assay for analysis of nine single nucleotide polymorphisms related to antigens that are difficult to assess using conventional serology.
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