Open AccessAF291 and AE391 single-chain antibodies recognize the HA tag by Western blotting
Author(s) -
Zsofia Keszei,
Didier Picard
Publication year - 2019
Publication title -
antibody reports
Language(s) - English
Resource type - Journals
ISSN - 2624-8557
DOI - 10.24450/journals/abrep.2019.e25
Subject(s) - blot , monoclonal antibody , antibody , recombinant dna , microbiology and biotechnology , biology , biochemistry , immunology , gene
The recombinant antibodies AF291and AE391 against the standard HA tag detect an HA-tagged protein exogenously expressed in human cells by Western blotting. However, similarly to one of the original monoclonal antibodies, they also recognize a number of unrelated endogenous proteins.
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