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Surface Plasmon Resonance (SPR) instrument is an important tool in the field of biomolecular interaction studies. The SPR instrument is basically an optical measurement technique and its construction is very complex. The construction of the SPR instrument is not that much easy because it is an integration of optics, mechanical, electronics and electrical. However, the instrument operation depends on the mechanical movement of the resonance angle. Based on the mechanical aspects the instrument is constructed in different aspects for better generation of SPR signals. To generate SPR signal the optical prism with a sensor is rotated by the stepper motor for resonance angle and the signals are collected at the detector. The optical setup is rotation by Ө-2Ө for optimal to produce the effective SPR signals. This paper describes the fabrication and working of SPR instrument based on Ө-2Ө.

Design and Development of SPR Instrument Based On Ө-2Ө Mechanical Integration Technique