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The capabilities of laser analysis of total protein (TP) were studied. Semiconductor laser diode of 5-mW maximum power, (532) nm wavelength and (4) nm bandwidth is used as laser system (LS) to analyze and find the concentration of total protein (TP) in human blood serum. All the absorption spectra of TP were measured with a UV–VIS spectrophotometer. The spectral plot showed that the maximum absorbance of (TP) is at the range (520-580nm) and the peak at (546) nm .The (LS) measurements included the study of absorbance (A) of (TP) as a function of cuvet thickness (t), transmittance (T) as a function of (t) and (A) as a function of laser power. To ascertain our calculations, the results have been compared with the results of spectrophotometer. The (RSD %) values are about (0.67-17.18).

Determination of total protein in human blood by laser