Investigation of Microsatellite Instability BAT 25 and BAT 26 in Breast Cancer Patients by Conventional PCR

Investigate the existence of microsatellite instability (MSI) in patients with invasive ductal breast carcinoma (IDC). Investigate the existence of microsatellite instability (MSI) in breast cancer of patients. DNA had been extracted from frozen tissue samples of breast cancer. This protocol done according to the kit manufacture’s manual of QIAamp DNA Mini kit from Qiagen – USA. All samples tested for MSI by singleplex PCR reactions using two microsatellite markers BAT 25 and BAT 26. PCR achieved in a final volume of 50 μl and after thermal cycles, gel visualization performed. PCR demonstrating microsatellite instability in 13 (26%) of the 50 breast cancer sample. Eight (16%) of 50 breast cancer sample were BAT 25 positive with a PCR product size of 124 bp, while 5 (10%) of 50 breast cancer sample were BAT 26 positive with a PCR product size 121bp. The result suggests strong evidence that microsatellite instability (MSI) at the BAT 25 and BAT 26 and have involved in the pathogenesis of the great majority of breast cancers.