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This study was carried out to determine the prevalence of infectious diseases in blood donors by individual donor nucleic acid testing (ID-NAT). Safe blood transfusion practices can save people from being exposed to the risk of transfusion transmittable infections. Serum samples from voluntary and replacement donors were tested for infectious diseases like Human Immunodeficiency Virus (HIV), Hepatitis C Virus (HCV) and Hepatitis B Virus (HBV) by ID-NAT and the results were analysed statistically. Majority of the donors were in the 3rd decade of life,15770 (59.6%); followed by in 4th decade of life, 5218 (19.7%).There were 24113(91.1%) males as compared to 2354(8.9%) female donors, with male-female ratio of 29.9:1. Replacement donations were 24583 (92.9%) and voluntary donations were 1884 (7.1%). The seroprevalence of HIV was 19(0.07%), HCV was 173(0.7%), HbsAg was 705(2.6%) and 25570(96.6%) donors were non reactive by ID-NAT. The sensitivity, specificity and diagnostic accuracy by ID-NAT was 99.2%, 96.7% and 99.8% respectively. ID-NAT is a very sensitive testing modality of infectious blood, which decreases the window period of infections, and thus enhances the safety of blood transfusion. Department of Pathology Jawaharlal Nehru Medical College, Faculty of Medicine, A.M.U., Aligarh India. Sumbul Warsi, Shafaque Zabin, Kafil Akhtar, SH Arif ERA’S JOURNAL OF MEDICAL RESEARCH SEROPREVALENCE OF INFECTIOUS DISEASES IN BLOOD DONORS BY IDNAT IN A TERTIARY CARE CENTRE IN NORTHERN INDIA VOL.7 NO.1 Original Article Page: 34 ERA’S JOURNAL OF MEDICAL RESEARCH, VOL.7 NO.1 Contact no: +91-9568467727 Dr. Kafil Akhtar Department of Pathology Jawaharlal Nehru Medical College, Faculty of Medicine, A.M.U., Aligarh India. Email: drkafilakhtar@gmail.com Address for correspondence Received on : xx-xx-xxxx Accepted on : xx-xx-xxxx