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Magnetic nanoparticles are widely used in areas such as biosensors, data storage devices, waste water treatment and biomedicine due to their biocompatibility and low toxicity. In this study, biosynthesis of the CoFe2O4 nanoparticle (NP) was carried out using the bark extract of Aesculus hippocastanum plant as a reducing and capping agent. Synthesized NPs were char-acterized by ultraviolet-visible spectrophotometer (Uv-Vis.), Dynamic Light Scattering (DLS), zeta load, X-Ray Dust Diffraction (XRD), Fourier Transformed Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy, Raman, BET, and Thermogravimetric Thermal Analysis (TGA). The Lethal dose value (LD50) and the crude oil removal efficiency were examined. The characteristic light absorption of the CoFe2O4NP has been measured at 330 nm, surface charge of +14.4 mV, mean size of 75 nm and effective diameter of 432 nm. The XRD analy-sis revealed that the particle structure was in the cubic spinel structure with functional groups bound by FT-IR analysis and Raman data. It has been noted that the CoFe2O4NP, which was synthesized by biological method, can remove a 78.5% of the crude oil from the contaminat-ed water. It has been determined that the NP’s have a low toxic effect on D. magna (LD50= 728.267 ppm). In this study, it has been suggested that the CoFe2O4 NP with A. hippocasta-num bark extract could be synthesized by a cheap, relatively easy and environmentally friendly method which used for purification of contaminated water bodies.

Biosynthesis , Characterization and Removal Efficiency for Petroleum Leakage of the Cofe2o4 Nanoparticles