In Vivo Monitoring of Parathyroid Hormone Treatment after Myocardial Infarction in Mice with [ 68 Ga]Annexin A5 and [ 18 F]Fluorodeoxyglucose Positron Emission Tomography | Zendy

Sebastian Lehner | Zendy; Andrei Todica | Zendy; Yordan Vanchev | Zendy; Christopher Uebleis | Zendy; Hao Wang | Zendy; Tanja Herrler | Zendy; Carmen Wängler | Zendy; Paul Cumming | Zendy; Guido Böning | Zendy; Wolfgang M. Franz | Zendy; Peter Bartenstein | Zendy; Marcus Hacker | Zendy; Stefan Brunner | Zendy

Open Access

In Vivo Monitoring of Parathyroid Hormone Treatment after Myocardial Infarction in Mice with [ ⁶⁸ Ga]Annexin A5 and [ ¹⁸ F]Fluorodeoxyglucose Positron Emission Tomography

Author(s) -

Sebastian Lehner,

Andrei Todica,

Yordan Vanchev,

Christopher Uebleis,

Hao Wang,

Tanja Herrler,

Carmen Wängler,

Paul Cumming,

Guido Böning,

Wolfgang M. Franz,

Peter Bartenstein,

Marcus Hacker,

Stefan Brunner

Publication year - 2014

Publication title -

molecular imaging

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.815

H-Index - 60

eISSN - 1536-0121

pISSN - 1535-3508

DOI - 10.2310/7290.2014.00035

Subject(s) - medicine , tunel assay , annexin , annexin a5 , positron emission tomography , parathyroid hormone , in vivo , apoptosis , nuclear medicine , infarction , pathology , ex vivo , myocardial infarction , endocrinology , urology , staining , chemistry , immunohistochemistry , biology , calcium , biochemistry , microbiology and biotechnology

[68Ga]Annexin A5 positron emission tomography (PET) reveals the externalization of phosphatidylserine as a surrogate marker for apoptosis. We tested this technique for therapy monitoring in a murine model of myocardial infarction (MI) including parathyroid hormone (PTH) treatment. MI was induced in mice, and they were assigned to the saline or the PTH group. On day 2, they received [68Ga]annexin A5 PET or histofluorescence TUNEL staining. Mice had 2-deoxy-2-[18F]fluoro-d-glucose (FDG)-PET examinations on days 6 and 30 for calculation of the left ventricular ejection fraction and infarct area. [68Ga]Annexin A5 uptake was 7.4 ± 1.3 %ID/g within the infarction for the controls and 4.5 ± 1.9 %ID/g for the PTH group (p = .013). TUNEL staining revealed significantly more apoptotic cells in the infarct area on day 2 in the controls (64 ± 9%) compared to the treatment group (52 ± 4%; p = .045). FDG-PET revealed a significant decrease in infarct size in the treatment group and an increase in the controls. Examinations of left ventricular ejection fraction on days 6 and 30 did not reveal treatment effects. [68Ga]Annexin A5 PET can detect the effects of PTH treatment as a marker of apoptosis 2 days after MI; ex vivo examination confirmed significant rescue of myocardiocytes. FDG-PET showed a small but significant reduction in infarct size but no functional improvement.

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