Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain | Zendy

Alessandro Luchetti | Zendy; Davide Milani | Zendy; Francesca Ruffini | Zendy; Rossella Galli | Zendy; Andrea Falini | Zendy; Angelo Quattrini | Zendy; Giuseppe Scotti | Zendy; Giacomo P. Comi | Zendy; Gianvito Martino | Zendy; Roberto Furlan | Zendy; Letterio S. Politi | Zendy

Open Access

Monoclonal Antibodies Conjugated with Superparamagnetic Iron Oxide Particles Allow Magnetic Resonance Imaging Detection of Lymphocytes in the Mouse Brain

Author(s) -

Alessandro Luchetti,

Davide Milani,

Francesca Ruffini,

Rossella Galli,

Andrea Falini,

Angelo Quattrini,

Giuseppe Scotti,

Giacomo P. Comi,

Gianvito Martino,

Roberto Furlan,

Letterio S. Politi

Publication year - 2012

Publication title -

molecular imaging

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.815

H-Index - 60

eISSN - 1536-0121

pISSN - 1535-3508

DOI - 10.2310/7290.2011.00032

Subject(s) - monoclonal antibody , superparamagnetism , antibody , cd3 , biophysics , chemistry , iron oxide nanoparticles , in vivo , antigen , nuclear magnetic resonance , microbiology and biotechnology , iron oxide , pathology , materials science , biology , immunology , medicine , physics , magnetization , organic chemistry , quantum mechanics , magnetic field , cd8

We investigated the potential of antibody-vectorialized superparamagnetic iron oxide (SPIO) particles as cellular specific magnetic resonance contrast agents to image lymphocyte populations within the central nervous system (CNS), with the final goal of obtaining a reliable tool for noninvasively detecting and tracking specific cellular populations in vivo. We used superparamagnetic particles bound to a monoclonal antibody. The particle is the contrast agent, by means of its T2* relaxation properties; the antibody is the targeting vector, responsible for homing the particle to target a surface antigen. To investigate the efficiency of particle vectorialization by these antibodies, we compared two types of antibody-vectorialized CD3-specific particles in vivo. We successfully employed vectorialized SPIO particles to image B220+ cells in a murine model of B-cell lymphoma. Likewise, we were able to identify CD3+ infiltrates in a murine model of multiple sclerosis. The specificity of the technique was confirmed by immunohistochemistry and electron microscopy of corresponding sections. Our findings suggest that indirect binding of the antibody to a streptavidinated particle allows for enhanced particle vectorialization compared to covalent binding of the antibody to the particle

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