Open AccessColor Transformation and Fluorescence of Prussian Blue–Positive Cells: Implications for Histologic Verification of Cells Labeled with Superparamagnetic Iron Oxide Nanoparticles
Author(s) -
Joseph A. Frank,
Heather Kalish,
Elaine Jordan,
Stasia A. Anderson,
Edyta Pawelczyk,
Ali S. Arbab
Publication year - 2007
Publication title -
molecular imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.815
H-Index - 60
eISSN - 1536-0121
pISSN - 1535-3508
DOI - 10.2310/7290.2007.00014
Subject(s) - prussian blue , fluorescence , superparamagnetism , fluorescence microscope , iron oxide , microscopy , iron oxide nanoparticles , nanoparticle , stain , materials science , biophysics , chemistry , staining , nuclear magnetic resonance , pathology , nanotechnology , optics , biology , medicine , magnetization , physics , electrode , quantum mechanics , magnetic field , metallurgy , electrochemistry
Superparamagnetic iron oxide (SPIO) nanoparticles, either modified or in combination with other macromolecules, are being used for magnetic labeling of stem cells and other cells to monitor cell trafficking by magnetic resonance imaging (MRI) in experimental models. The correlation of histology to MRI depends on the ability to detect SPIO-labeled cells using Prussian blue (PB) stain and fluorescent tags to cell surface markers. Exposure of PB-positive sections to ultraviolet light at a wavelength of 365 nm commonly used fluorescence microscopy can result in color transformation of PB-positive material from blue to brown. Although the PB color transformation is primarily an artifact that may occur during fluorescence microscopy, the transformation can be manipulated using imaging process software for the detection of low levels of iron labeled cells in tissues sample
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