A Simple Method for Cloning Giardia duodenalis from Cultures and Fecal Samples | Zendy

N. Binz | Zendy; R.C.A. Thompson | Zendy; Bruno P. Meloni | Zendy; Alan J. Lymbery | Zendy

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A Simple Method for Cloning Giardia duodenalis from Cultures and Fecal Samples

Author(s) -

N. Binz,

R.C.A. Thompson,

Bruno P. Meloni,

Alan J. Lymbery

Publication year - 1991

Publication title -

journal of parasitology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.467

H-Index - 92

eISSN - 1937-2345

pISSN - 0022-3395

DOI - 10.2307/3283171

Subject(s) - biology , cloning (programming) , feces , giardia , microbiology and biotechnology , computer science , programming language

Using a novel method for cloning Giardia duodenalis from cultures and fecal samples, 47 clones from 7 isolates were established in vitro. Average colony-forming efficiency in established cultures was 43.2% compared to 11.2% when cloning directly from excystation. The highest success rate of cloning was found with the Portland (P1, ATCC No. 30888) isolate, with a colony-forming efficiency of 92.7%. Cloned and parent populations were compared over a range of 13 enzymes using starch gel electrophoresis. No genetic difference was found between any of the clones and the parent isolates.

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