Degradation of linuron in soil by two fungal strains

Two fungal strains were applied to soil polluted with herbicide in order to determine their degradation potential. Three experimental setups were used. In the first setup, the soil in pots was contaminated by linuron in final concentration of 1 ppm. Suspensions of Phanerocheate chrysosporium and Trichoderma asperellum were applied separately or in combination. Tomato plantlets were transplanted and chlorophyll content in their leaves was determined at two time points during plant growth. In the second setup in pots, the final concentration of linuron was lower, 0.45 ppm. In the third setup 0.1 ppm of linuron was applied in the field plot. Plantlets of lettuce were transplanted and chlorophyll content was measured as indicator of plant stress. The content of linuron in soil was determined by HPLC. The applied fungal strains significantly reduced toxic effect of 0.45 ppm linuron on plants, which was not the case for 1 ppm linuron. Both fungi, applied separately or in combination, were effective in decreasing the linuron content in the soil. However, in field conditions the combination of both fungi was the most effective.