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Analysis of feed for the presence of fungi and mycotoxins is a request  necessary to meet in order to ensure a healthy and economical production in  livestock. These tests are related to legal regulation which prescribes the  maximum legislated content (MLC), both for the presence of mycotoxins and the  total number of fungi in certain feeds. Health problems that can occur during  the production of animals are sometimes caused by the presence of mycotoxins  in the feed. Laboratory testing is a good practice to confirm a suspicion,  and allows timely treatment of contaminated feed. Potential problems arise  under circumstances when there is a clinical outcome of mycotoxicosis and  animal and laboratory findings suggest that the obtained values are below the  level that is within the MLC. For these reasons, the subject of our research  was to investigate the occurrence of mycotoxins and mold in feed, as well as  the clinical presentation for animals that were fed with the feed with  allowed values of these agents according to the recommended levels. The aim  of this paper was to highlight the problems associated with clinical  correlation of sick animals and laboratory findings, and suggest their  overcoming. In the period of one year, a total of 176 samples of feed  (complete mixture for broilers, corn and soy products) were examined for the  presence of fungi, 106 samples were examined for the presence of mycotoxins  and 26 flocks of broilers and turkeys were clinically observed. Standard  methods were used for isolation of molds and the ELISA test was used for the  detection of mycotoxins. Clinical and pathomorphological observation of the  flocks was done to determine the natural indicators of production. Studies  indicated a problem because clinical and pathomorphological findings in some  cases were not correlated with laboratory findings of molds and mycotoxins in  the feed, and in some cases it did not necessarily mean that the animals were  healthy. Synergism and cumulative effects of mycotoxins, on the one hand, and  the characteristics of each species and product category on the other hand,  can create specific circumstances that can lead to disease and can increase  even though the values prescribed by legislation have been met. [Projekat  Ministarstva nauke Republike Srbije, br. TR 031071

Correlation between the limit values of laboratory and clinical mycotoxicosis