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Background/Aim. A wide range of resorbable and nonresorbable membranes have  been investigated over the last two decades. The barrier membrane protects  the defect from ingrowth of soft tissue cells and allows bone progenitor  cells to develop bone within a blood clot that is formed beneath the barrier  membrane. The membranes are applied to reconstruct small bony defect prior to  implantation, to cover dehiscences and fenestrations around dental implants.  The aim of this study was to evaluate the influence of human resorbable  demineralized membrane (RHDM) thickness on bone regeneration. Methods. The  experiment, approved by Ethical Committee, was performed on 6 dogs and  conducted into three phases. Bone defects were created in all the 6 dogs on  the left side of the mandible, 8 weeks after extraction of second, third and  fourth premolars. One defect was covered with RHDM 100 μ thick, one with RHDM  200 μ thick, and the third defect left empty (control defect). The  histopathological analysis was done 2, 4 and 6 months after the surgery. In  the third phase samples of bone tissue were taken and subjected to  histopathological analysis. Results. In all the 6 dogs the defects treated  with RHDM 200 μ thick showed higher level of bone regeneration in comparison  with the defect treated with RHDM 100 μ thick and especially with empty  defect. Conclusion. Our results demonstrated that the thicker membrane showed  the least soft tissue ingrowths and promoted better bone formation at 6  months compared with a thinner one

The impact of thickness of resorbable membrane of human origin on the ossification of bone defects: A pathohistologic study