Reliability of Cobas Amplicor PCR test in detection of Mycobacterium tuberculosis in respiratory and nonorespiratory specimens | Zendy

Zorica Lepšanović | Zendy; Dejana Savić | Zendy; Branka Tomanović | Zendy

Open Access

Reliability of Cobas Amplicor PCR test in detection of Mycobacterium tuberculosis in respiratory and nonorespiratory specimens

Author(s) -

Zorica Lepšanović,

Dejana Savić,

Branka Tomanović

Publication year - 2009

Publication title -

vojnosanitetski pregled

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.123

H-Index - 19

eISSN - 2406-0720

pISSN - 0042-8450

DOI - 10.2298/vsp0912992l

Subject(s) - mycobacterium tuberculosis , polymerase chain reaction , acid fast , microbiology and biotechnology , tuberculosis , nucleic acid amplification tests , sputum , medicine , mycobacterium , bacilli , roche diagnostics , respiratory system , bacteria , virology , biology , pathology , gene , biochemistry , genetics , chlamydia trachomatis

Traditional methods for detection of mycobacteria, such as microscopic examination for the presence of acid-fast bacilli and isolation of the organism by culture, have either a low sensitivity and/or specificity, or take weeks before a definite result is available. Molecular methods, especially those based on nucleic acid amplification, are rapid diagnostic methods which combine high sensitivity and high specificity. The aim of this study was to determine the usefulness of the Cobas Amplicor Mycobacterium tuberculosis polymerase chain reaction (CA-PCR) assay in detecting the tuberculosis cause in respiratory and nonrespiratory specimens (compared to culture).

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