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Babesiosis of domestic animals is a vector transmissible and clinically   significant disease, caused by protozoa of genus Babesia and Theileria.   Possible causative agents for this disease in dogs in Europe are: Babesia   canis, B. gibsoni, B. vogeli and B. microti-like. Diagnostics of babesiosis   of dogs was for a long time based on the visual inspection of stained blood   smear under a microscope, while today there have been increasingly used   molecular methods of detection in precise, species diagnostics. The   objective of this work was molecular detection of the cause of babesiosis of   dogs in the ticks sampled from asymptomatic dogs in the region of some   Belgrade municipalities, all for better understanding of epizootiological   situation. From three sites in Belgrade, there were collected 49 ticks,   sampled from the dogs with no symptoms. There was carried out the   determination of the ticks, and after that, DNA was isolated for molecular   examination. First, there was performed Polymerase Chain Reaction (PCR),   for determining the species of the genus Babesia, and after that there was   also carried out the determining of polymorphism in the length of   restriction fragments (RFLP) for the purpose of the causative agent species   determination. Out of the total number of the examined ticks, 18,34% were   positive on Babesia spp. By RFLP method, in two cases (4,08%) B. Gibsoni   was identified, while in 7 cas­es (14,92%) there were no restriction sites   for the used enzymes, what suggests that most likely it was B. canis. The   ticks positive on the cause of babesiosis were: Dermacentor reticulatus (4   cases), Rhipicephalus sanguineus (4 cases) i Ixodes ricinus (1 case). This   work confirms the presence of Babesia spp. in the ticks sampled from   asmptomatic dogs on the teritory of Belgrade as well as the significance of   PCR-RFLP method in diagnostics and identification of the causative agent of   babesiosis in dogs. For the first time in Serbia, there was determined the   presence of B. gibsoni in ticks (Species Rhipicephalus sanguineus

Molecular detection of Babesia spp. in ticks sampled from asymptomatic dogs in the area of some Belgrade municipalities