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The objective of our work was to investigate some biological characteristics   of purified glycoprotein subunits of Newcastle disease virus strain   PHY-LMV.42 isolated from pigeons for the purpose of vaccine production.   PHY-LMV.42 strain of Newcastle disease virus was multiplied by successive   passages in embryonated eggs and identified by the methods of Reverse   transcriptase PCR and Real-Time PCR along with F gene sequencing. Proving the   presence of HN and F antigene in the virus subunits samples was carried out   by hemagglutination inhibition method with referent immune sera. Biochemical   characterization of glycoprotein subunits was performed by SDS-PAGE method as   well as liquid chromatography with mass spectrometry (LC ESI-TOF-MS/MS).   Testing for the virus subunits immunogenicity was carried out in biological   experiment on 75 laying hens Tetra-SSL and 25 chickens Isa Brown by inducing   an artificial infection with Hertz 33 strain of the virus. Low concentrations   of the virus antigens of 0.36 mg/ml along with glycoprotein fractions of 77 i   58 kDa manifested a strong hemagglutination activity of 4096 HJ/0,1ml. The   subunit vaccines of 256 and 128 HJ/0.5 ml induced a protective immune   response in all the vaccinated animals. Based on the obtained results it can   be concluded that low concentrations of purified virus subunits of PHY-LMV.42   strain can be used for preparing of effective vaccines. [Projekat   Ministarstva nauke Republike Srbije, br. TR 31008: Development and   application of molecular methods based on polymerase chain reaction (PCR) in   quick and direct identification of Newcastle disease virus strains and   investigation of immunogenicity of subunit vaccine prepared of their   antigens

Examination of some biological properties of glycoprotein subunits of PHY-LMV.42 strain of Newcastle disease virus