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The objective of these investigations was to check on laboratory white mice   the protective effect of an inactivated bivalent vaccine prepared from   serotypes 1/2a and 4b L. monocytogenes. Following verification of the   sterility and toxicity of the prepared vaccine, the mice were divided into 6   groups with 10 animals in each group. The first and second group of mice were   administered the vaccine without saponin (vaccine A) and the third and fourth   group the vaccine with saponin (vaccine B). Mice of the fifth and the sixth   group were not vaccinated and served as a negative control. Two weeks   following vaccination, the experimental groups were revaccinated, with the   exception of the two control groups. Two weeks following revaccination, all   groups were artificially infected with serotypes 1/2a and 4b L.   monocytogenes. During the course of the investigations (60 days) a total of 4   mice died in the vaccinated groups. Mice of the control groups started dying   after day 7, and the last mouse in these groups died 14 days after the   infection. Examinations of preparations of parenchymatous organs of the dead   mice stained according to Gram proved the presence of L. monocytogenes.   Homogenates of parenchymatous organs were sown on tryptose agar for   reisolation and a pure culture of L. monocytogenes was obtained. Through the   use of specific antiserums, serotypes 1/2a and 4b were confirmed. Considering   the total number of vaccinated mice in the experiment and the percent deaths   (10%), it can be said that the investigated vaccine with saponin had a   satisfactory protective effect. [Projekat Ministarstva nauke Republike   Srbije, br. TR-20142 i TR-31088

Investigations of protective effects of bivalent inactivated vaccine prepared from serotypes 1/2a and 4b Listeria monocytogenes on mice