The effect of irrigating solutions on the Ca/P ration in root dentine

The aim of this study was to evaluate the effect of Na-EDTA and NaOCl in mineral contents in root dentine using SEM and EDS. Material and method: Twenty-two specimens of the middle radicular third obtained from human first molars, extracted for orthodontic reasons, were examined. Specimens were polished and divided into four groups. The first group was treated with saline and used as control. The second group was treated with Na-EDTA for one minute, followed by saline irrigation. The third group was treated with combination of Na-EDTA and NaOCl and the last, fourth group was treated with NaOCl only. Results have shown that Na-EDTA combined with NaOCl as the final flush and NaOCl alone, have significantly influenced changes in the Ca/P ration of the root dentine. The use of NaOCl as the final flush altered the effectiveness of chelating agents in root dentine.