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Pyrolysis technology for producing biochar and bio-oils can be used as a  potential alternative to make biopesticides, which are urgently needed in  integrated pest management (IPM). Insecticidal activity of three components  of bio-oils: aqueous, organic and their mixture, was evaluated individually  and with three different entomopathogens: the baculovirus Agrotis ipsilon  nucleopolyhedrovirus (AgipMNPV), bacterium Bacillus thuringensis var.  kurstaki (Bt) and fungus Beauveria bassiana (Bb) against black cutworm,  Agrotis ipsilon (Hufnagel). The effect of alkaline conditions of spray-dried  biochar was studied simultaneously with the microbial pathogens. Our  bioassay results indicated that the organic bio-oil phase was the most  active, causing 100% mortality after 24 h, when the median lethal toxicity  values LC50s (mg/ml) were found. However, the bio-oil fractions applied  alone tended to cause higher mortality of the exposed larvae than did either  mix with the microbial agents. Also, the results revealed that maximum  mortalities were found in spray-dried formulations made with biochar at pH  7.1. It was concluded that pyrolysis oils are effective insecticides, and  biochar could be a useful additive in production and formulation of  biopesticides. This interesting finding further promotes the use of  pyrolysis bio-oils and biochar compounds as eco-friendly alternatives to  replace conventional pesticides.

pesticidi i fitomedicina

Insecticidal activity of bio-oils and biochar as pyrolysis products and their combination with microbial agents against agrotis ipsilon (Lepidoptera: Noctuidae)