The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia | Zendy

Dragana Milošević | Zendy; Maja Ignjatov | Zendy; Zorica Nikolić | Zendy; Ivana Stanković | Zendy; A. Bulajić | Zendy; Ana MarjanovićJeromela | Zendy; B. Krstić | Zendy

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The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia

Author(s) -

Dragana Milošević,

Maja Ignjatov,

Zorica Nikolić,

Ivana Stanković,

A. Bulajić,

Ana MarjanovićJeromela,

B. Krstić

Publication year - 2016

Publication title -

pesticidi i fitomedicina

Language(s) - English

Resource type - Journals

eISSN - 2406-1026

pISSN - 1820-3949

DOI - 10.2298/pif1602037m

Subject(s) - turnip mosaic virus , biology , myzus persicae , brassica , genbank , cauliflower mosaic virus , virus , virology , plant virus , botany , veterinary medicine , potyvirus , gene , aphid , genetically modified crops , genetics , medicine , transgene

A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates. [Projekat Ministarstva nauke Republike Srbije, br. TR 31025 i br. III-43001]

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