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A total of 86 oilseed rape samples from six crops in different localities  were collected during 2014 and analyzed for the presence of Turnip yellows  virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV)  using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was  serologically detected in 60 collected samples (69.77%), and none of the  samples tested were positive for CaMV and TuMV. Six selected TuYV isolates  were successfully transmitted by Myzus persicae to three test plants,  confirming the infectious nature of the disease. In the selected  ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR  and sequencing. A comparison of the obtained sequence with those available in  GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis  of P0 gene sequence data for a subset of these isolates showed they clustered  with the known TuYV and were distinct from Beet western yellows virus (BWYV)  isolates. [Projekat Ministarstva nauke Republike Srbije, br. TR 31025 i br.  III-43001]

The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia