Dissipation rate of acetamiprid in sweet cherries

Degradation of acetamiprid in sweet cherry samples was evaluated at several intervals\udfrom the product application until the end of the pre-harvest interval. An orchard\udof sweet cherries located at Stepanovićevo village near Novi Sad was used in this study.\udAcetamiprid was applied according to the manufacturer’s recommendation for protecting\udsweet cherries from their most important pests. Sweet cherry fruit samples were collected\udat eight intervals: immediately after acetamiprid application and 2, 4, 6, 8, 10, 12\udand 14 days after application. The extraction of acetamiprid from sweet cherry samples\udwas performed using a QuEChERS-based method. Determination was carried out using\udan HPLC-UV diode array detection system (Agilent 1100, United States) with an Agilent\udZorbax Eclipse C18 column (50 mm × 4.6 mm internal diameter, 1.8 μm particle size).\udThe method was subjected to a thorough validation procedure. The recovery data were\udobtained by spiking blank sweet cherry samples at three concentration levels (0.1-0.3 mg/\udkg), yielding 85.4% average recovery. Precision values expressed as relative standard deviation\ud(RSD) were below 1.61% for the intraday precision. Acetamiprid showed linear calibrations\udfrom 0.05 to 2.5 μg/ml with correlation coefficient (R2) of 0.995%. The limit of\uddetection and limit of quantification were found to be 5 μg/kg and 14 μg/kg, respectively.\udThe validated method was applied in the analysis of acetamiprid in sweet cherry samples.\udDuring the study period, the concentration of acetamiprid decreased from 0.529\udmg/kg to 0.111 mg/kg. The content of acetamiprid in sweet cherry samples at the end\udof the pre-harvest interval was below the maximum permissible level specified by the\udSerbian and EU MRLs