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Brassicas form the most important group of vegetable crops in Montenegro. The  cabbage (Brassica oleracea var. capitata) is most commonly grown, although  other brassicas, particularly kale, Brussels sprout, cauliflower and  broccoli, have been increasingly produced since recently. One of the  specialties of vegetable production in Montenegro is growing of collard  (Brassica oleracea var. acephala), which is the simplest variety of the  Brassica oleracea species and in the nearest relation with their wild  ancestor - the sylvestris variety. Diseases are the main restrictive factors  for successful production of these vegetables. Susceptibility of the  cultivars and inadequate control often result in more or less damaged crops  in some plots. Causal agents of brassica diseases, especially bacterial, have  not been investigated in Montenegro until 2009. Since the symptoms observed  in 2009 were „V” shaped leaf edge necrosis and black rot of vascular tissue,  it was assumed that they were caused by plant pathogenic bacterium  Xanthomonas campestris pv. campestris. Samples of the infected plants were  collected from different localities in Montenegro. Isolation and  identification of the bacterium were performed using laboratory methods  according to Schaad (1980), Lelliott and Stead (1987) and Arsenijevic (1997).  Examination of chosen bacterial isolates was conducted using both, classical  bacteriological methods (examination of their pathogenic, morphological,  cultivation and biochemical and physiological characteristics), and ELISA  test. The obtained results confirmed the presence of X.campestris pv.  campestris (Pammel, 1895) Dowson 1939, on cabbage, kale, broccoli and collard  in Montenegro. This is the first experimental evidence that collard is the  host of X. campestris pv. campestris in Montenegro.

Occurrence of Xanthomonas campestris pv. campestris (Pammel, 1895) Dowson 1939, on brassicas in Montenegro