Open AccessTolerance of spring and winter wheats to herbicide in in vitro culture
Author(s) -
Ankica KondićŠpika,
Radivoje Jevtić
Publication year - 2002
Publication title -
pesticidi
Language(s) - English
Resource type - Journals
eISSN - 2406-0844
pISSN - 0352-9029
DOI - 10.2298/pif0204125k
Subject(s) - biology , spring (device) , callus , agronomy , horticulture , mechanical engineering , engineering
Spring and winter wheat varieties have been compared for tolerance to tribenuron in the zygous embryo culture. Four randomply selected varieties of each spring and winter wheat were used for embryo isolation. The embryos were grown for 30 days in a modified MS (Murashige and Skoog, 1962) medium to which tribenuron was added in three different concentrations (18.75 mgl~ 37.50 mgl", 56.25 mgl 1). The percentage of surviving calli and the percentage reduction of callus fresh mass in relation to the control variant served as parameters of tolerance. All of the tested parameters indicated that the winter wheats had a higher level of tolerance to the herbicide than the spring wheats.