Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India) | Zendy

Sancho Rajan | Zendy; Anwesha Pattanaik | Zendy; Venkatesh Kumaresan | Zendy; Prasanth Bhatt | Zendy; Gunasekaran Senthilarasu | Zendy; Jesu Arockiaraj | Zendy; Mukesh Pasupuleti | Zendy; Vladimir Beškoski | Zendy; Paromita Chakraborty | Zendy

Open Access

Characterization of some naphthalene using bacteria isolated from contaminated Cooum Riverine sediment of the Bay of Bengal (India)

Author(s) -

Sancho Rajan,

Anwesha Pattanaik,

Venkatesh Kumaresan,

Prasanth Bhatt,

Gunasekaran Senthilarasu,

Jesu Arockiaraj,

Mukesh Pasupuleti,

Vladimir Beškoski,

Paromita Chakraborty

Publication year - 2018

Publication title -

journal of the serbian chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.227

H-Index - 45

eISSN - 1820-7421

pISSN - 0352-5139

DOI - 10.2298/jsc180724088r

Subject(s) - 16s ribosomal rna , naphthalene , pseudomonas , microorganism , sediment , bioremediation , bacteria , microbiology and biotechnology , biology , environmental chemistry , chemistry , organic chemistry , genetics , paleontology

Microorganisms capable of using naphthalene as the sole carbon source were isolated from the contaminated sediment of CooumRiver. Twenty one isolates were recovered and nine were selected for enrichment due to differences in their morphological characteristics. Out of nine isolates, only four (NS3-SRMND14B, NS14-SRMND14A, NS15-SRMND14D and NS19- -SRMND14E) were capable of completely utilizing naphthalene as the sole source of carbon in carbon free minimal medium (CFMM) supplemented with naphthalene. 16S rDNA sequencing showed that all the four isolates were distantly related to each other and belongs to Bacillus sp. (NS3-SRMND14B), Pseudomonas sp. (NS14-SRMND14A), Cellulosimicrobium sp. (NS15-SRMND14D) and Sphingobacterium sp. (NS19-SRMND14E), respectively. Based on the phylogenetic analysis of 16S rDNA sequencing, the isolate Sphingobacterium sp. (NS19-SRMND14E) has been identified as novel strain. Polymerase chain reaction (PCR) technique showed the presence of naphthalene dioxygenase (ndo) gene responsible for naphthalene degradation only in the Pseudomonas sp. (NS14-SRMND14A). We observed that the ndo gene is not the only gene responsible for naphthalene degradation. Based on our study, the indigenous microorganisms isolated from Cooum Riverine sediment can be used for bioremediation of the polluted sediment along theBay of Bengal.

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