3-hydroxyflavone-bovine serum albumin interaction in Dextran medium | Zendy

Mariana Voicescu | Zendy; Sorana Ionescu | Zendy

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3-hydroxyflavone-bovine serum albumin interaction in Dextran medium

Author(s) -

Mariana Voicescu,

Sorana Ionescu

Publication year - 2014

Publication title -

journal of the serbian chemical society

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.227

H-Index - 45

eISSN - 1820-7421

pISSN - 0352-5139

DOI - 10.2298/jsc140425075v

Subject(s) - circular dichroism , bovine serum albumin , chemistry , fluorescence , tryptophan , dextran , fluorescence spectroscopy , intramolecular force , chromatography , biophysics , crystallography , biochemistry , stereochemistry , amino acid , physics , quantum mechanics , biology

Spectroscopic analysis of a bioactive flavonol, 3-Hydroxyflavone (3-HF), in systems based on Dextran 70 (Dx70) (an important bio-relevant polysacharide) and Bovine Serum Albumin (BSA) (a carrier protein), have been studied by fluorescence and circular dichroism. Changes produced by different concentrations of Dx70 on the fluorescent characteristics of 3-HF, and on the excited - state intramolecular proton transfer (ESIPT) process were studied. The influence of 3-HF binding and of Dx70 on the secondary structure of BSA were investigated by circular dichroism spectroscopy. The influence of temperature (30-80°C range) on the intrinsic Tryptophan fluorescence in 3-HF/BSA/Dx70 systems, was investigated. The results are discussed with relevance to 3-HF as a sensitive fluorescence probe for exploring flavone-protein interaction in plasma expander media and also for its biological evaluation

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