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The essential oil obtained from Citrus wilsonii Tanaka leaves with   hydrodistillation was investigated by GC and GC-MS. The main components of   the essential oil were identified to be citronellol (16.94%), nerol acetate   (10.35%), γ-terpinen (9.85%), citronellal (9.36%) and β-pinene (6.72%). Among   them, the four active constituents, predicted with a bioactivity-test, were   isolated and identified as citronellol, γ-terpinene, nerol (neryl) acetate   and β-pinene. It was found that the essential oil of C. wilsonii leaves and   isolated compounds possessed fumigant and contact toxicity against Tribolium   castaneum adults. The essential oil and γ-terpinen showed strong fumigant   toxicity against T. castaneum (LC50 = 8.18 and 4.09 mg L-1). Repellency of   the crude oil and active compounds was also determined. Citronellol, neryl   acetate and β-pinene were strongly repellent (100%, 86% and 92%,   respectively, at 78.63 nL cm-2, after 2 h treatment) against T. castaneum.   The essential oil and citronellol exhibited the same level of repellency   compared with the positive control, DEET. The results indicate that the   essential oil of C. wilsonii leaves and its active compounds had the   potential to be developed as natural fumigants, insecticides and repellents   for control of T. castaneum

Chemical constituents and biological activities against Tribolium castaneum (Herbst) of the essential oil from Citrus wilsonii leaves