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A simple micelle-mediated phase separation method has been developed for the  pre-concentration of trace levels of iron as a prior step to determination by  flame atomic absorption spectrometry (FAAS). The method is based on the cloud  point extraction (CPE) of iron using non-ionic surfactant  polyethyleneglycolmono-p-nonylphenylether (PONPE 7.5) without adding any  chelating agent. Several variables affecting the extraction efficiency were  studied and optimized utilizing central composite design (CCD) and three  levels full factorial design. Under the optimum conditions, the limit of  detection (LOD), limit of quantification (LOQ) and pre-concentration factor  were 1.5 μg L-1, 5.0 μg L-1 and 100, respectively. The relative standard  deviation (RSD) for six replicate determinations at 50 μg L−1 Fe(III) level  was 1.97%. The calibration graph was linear in the rage of 5-100 μg L-1, with  a correlation coefficient of 0.9921. The developed method was validated by  the analysis of two certified reference materials and applied successfully to  the determination of trace amounts of Fe(III) in water and rice samples

journal of the serbian chemical society

Optimization of cloud point extraction procedure with response surface methodology for quantification of iron by means of flame atomic absorption spectrometry